Cre- lox -Based System for Multiple Gene Deletions and Selectable-Marker Removal in Lactobacillus plantarum
نویسندگان
چکیده
منابع مشابه
Cre-lox-based system for multiple gene deletions and selectable-marker removal in Lactobacillus plantarum.
The classic strategy to achieve gene deletion variants is based on double-crossover integration of nonreplicating vectors into the genome. In addition, recombination systems such as Cre-lox have been used extensively, mainly for eukaryotic organisms. This study presents the construction of a Cre-lox-based system for multiple gene deletions in Lactobacillus plantarum that could be adapted for us...
متن کاملInducible gene targeting in mice using the Cre/lox system.
Molecular techniques now allow the design of precise genetic modifications in the mouse. Not only can defined nucleotide changes be engineered into the genome of the mouse, but genetic switches can be designed to target expression or ablation of any gene (for which basic molecular information is available) to any tissue at any defined time. These strategies promise to contribute substantially t...
متن کاملMarker removal in staphylococci via Cre recombinase and different lox sites.
Allelic replacement in staphylococci is frequently aided by antibiotic resistance markers that replace the gene(s) of interest. In multiply modified strains, the number of mutated genes usually correlates with the number of selection markers in the strain's chromosome. Site-specific recombination systems are capable of eliminating such markers, if they are flanked by recombinase recognition sit...
متن کاملModified gateway system for double shRNA expression and Cre/lox based gene expression
BACKGROUND The growing need for functional studies of genes has set the stage for the development of versatile tools for genetic manipulations. RESULTS Aiming to provide tools for high throughput analysis of gene functions, we have developed a modified short hairpin RNA (shRNA) and gene expression system based on Gateway Technology. The system contains a series of entry and destination vector...
متن کاملBroad-host-range cre-lox system for antibiotic marker recycling in gram-negative bacteria.
Complete genome sequences are now available for many bacterial species that lack sophisticated genetic tools. We describe the development of a broad-host-range cre-lox system that allows antibiotic marker recycling in a variety of gram-negative bacteria. This system consists of an allelic exchange vector bearing a kanamycin cassette flanked by loxP sites and a tetracycline-resistant IncP plasmi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2007
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.01473-06